Regulatory

Part:BBa_K511001:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

minCMV-1xCI434 Promoter MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This particular promoter has a propensity to mutate in cells that are not exceptionally recombination-deficient, and caution should be taken in producing both entry and expression vectors using this part.


Source

This promoter is a synthetic construct composed of the minimal sequence required for transcription from the immediate-early cytomegalovirus (CMV) promoter preceded by a binding site for the CI434 protein adapted from bacteriophage 434. Sincere thanks go to Noah Davidsohn for contributing this entry vector to the MIT iGEM 2011 team.

References